Katalog Numarası

101167

Synonyms

Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black

Description

Amido black 10 B (C.I.20470)

Overview

Amido black 10 B (C.I.20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

CAS Numarası

1064-48-8

EC number

213-903-1

Hill Formula

C₂₂H₁₄N₈Na₂O₉S₂

Molar Mass

616.5 g/mol

HS Code

3204 12 00

Structure formula Image